Phytochemical Investigation And Antibacterial Activity Of Root Extracts Of Rumex Nepalensis Spreng

Abstract

Rumex Nepalensis Spreng Belongs To Polygonaceae Family. Locally In Afan Oromo It Is Known As?�?Timiji?�? And In Amharic?�?Tult?�?. It Is Used Traditionally For The Treatment Of Various Ailments Including Pain, Inflammation, Bleeding, Constipation, Head Ache, Fever And Stomach Ulcer. Therefore, The Present Study Was Aimed To Investigate The Phytochemical Constituents, Isolating Compounds And Evaluating For Antibacterial Activities On The Solvent Extracts Of The Root Of R.Nepalensis Spreng. The Powdered And Dried Roots Of R.Nepalensis Spreng Was Successively Extracted With Equal Volume Of N-Hexane, Ethyl Acetate And Methanol By Maceration Methods In Increasing Polarity. The Solvents Were Evaporated Using Rotary Evaporator To Afford N-Hexane (1g), Ethyl Acetate (6.9g) And Methanol (70g) Crude Extracts Respectively. Structure Elucidation Was Done By Employing Some Physical Properties, Spectroscopic Methods And Comparing With Literature Data Reported For The Same Compounds. For Each Of The Crude Extracts, The Preliminary Phytochemical Screening Was Carried Out By Chemical Methods To Analyze The Presence Of Phytochemical Constituents Namely: Tannins, Saponins, Terpenoids, Flavonoids, Glycosides, Anthraquinones, Steroids, And Phenols But Alkaloids Were Absent In All Extracts. Silica Gel Column Chromatographic Separation Of The Ethyl Acetate Extract Afforded Oleic Acid (16), 4?��?Acetoxy-3?��?,4?��?-Diethoxy Epicatechin-3-O-Gallate Compound(17) And 4?��??��?-