Phytochemical Investigation On Leaves Of Leonot is Ocymifolia

Abstract

Leonotis ocymifolia belongs to the family Lamiaceae, in which the genus contains about 30 species. It is an indigenous to Eastern and Southern Africa, where people used locally for treatment of fever, intestinal parasites and headache. But, no detailed scientific investigation is available on the chemical composition of this plant. So, this research was aimed to isolate and characterize the chemical constituents from leaves of the plant. To achieve this objective, the leaves of the plant was extracted successively with petroleum ether, chloroform, chloroform/methanol, methanol. Phytochemical screenig was carried out on chloroform extract. The result indicated the presence of alkaloid, taninns, terpenoid and absence of sponnins. The chloroform extract was selected for further fractionation and prufication and subjected to column chromatography. The column was eluted with petroleum ether, methanol, chloroform with increasing polrity. Based on PTLC profile and one pure compound was obtaned from fr18 coded as LO-1 was characterized as1,2,3,5,6,7,8,8a octahydro-l-hydrox-5,5-dimethyl-2-methyiene-1((E)-5 methylhex-3-en-2-yl) naphthalen-4-yl acetate. Additionally, two compounds coded as LO-2 and LO-3 were isolated from the chloroform extract. However because of their low amount it was impossible to characterize their structures. The sturcure of the compound was characterized using spectroscopic techniques such as IR, 1H NMR,13C NMR, DEPT and UV spectral data.