Green Synthesis Of Iron Oxide Nanoparticles Using Ocimum Lamiifolioum For Anti- Bacterial Activities

Abstract

The progress of green chemistry in the synthesis of nanoparticles using plants has attracted a great attention nowadays due to its low cost, simple, non-toxic and environmental-friendly nature. The present study focuses on the green synthesis and characterization of Iron oxide nanoparticles using different ratios of ocimum Lamiifolium leaf extract for antibacterial application on the drug resistant bacteria. Iron (II) and Iron (III) hydrated chloride were used as precursor to synthesize Fe3O4 nanoparticles using the Ocimum Lamiifolium leaf extract. FeCl2.4H2O and FeCl3.6H2O used to synthesis Fe3O4 nanoparticles. The synthesized nanoparticles were characterized by advanced techniques like Thermo Gravimetric Analysis (TGA), X-Ray Diffraction (XRD) , Fourier Transform Infrared Spectroscopy (FT-IR), UVVisible spectroscopy, scanning electron microscopy (SEM) with EDS along with the evaluation of their antibacterial activity against gram positive and gram negative bacteria with a view to explore their pharmaceutical applications. Iron oxide nanoparticles were synthesized by co precipitation method using ferric chloride and NaOH as precursor to study the thermal stability of materials. The XRD analysis reveals all the synthesized particles have pure hexagonal phase with particle size in the range of 13 – 37 nm. The FT-IR analysis shows the interaction between the capping agents and the synthesized Fe3O4 nanoparticle. These Fe3O4-NPs fabricated through biosynthesis method, are promising candidate in various applications like biomedical and utilizing as recyclable magnetic nanocatalyst for organic reactions. The study on antibacterial activity of synthesized Fe3O4 NPs, against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa have better performance with the reference to the selected standards penicillin. The iron oxide nanoparticles showed their antibacterial properties on both gram positive and gram negative bacterial strains. As the diameter of the zone of inhibition is high, we can conclude that iron oxide is a very effective antibacterial agent.