Genetic Diversity Of Selected Accessions Of Groundnut (Arachis Hypogaea L.) In Ethiopia Using Inter Simple Sequence Repeat Marker

Abstract

Groundnut (Arachis Hypogaea L.) Belongs To The Family Leguminoseae And It Is The World?�?S 4th Most Important Source Of Edible Oil And 3rd Most Important Source Of Vegetable Protein. In Ethiopia Their Morphological Variations Have Already Been Documented But Molecular Variations Was Not Studied For This Valuable Crop. The Main Objective Of This Study Was To Determine The Genetic Diversity Of A. Hypogaea Accessions From Ethiopia Using Issr Marker. Using Four Issr Primers, 56 Reproducible Bands Were Generated Of Which 29 Were Polymorphic. The Band Size Ranged From 120 Bp To 1100 Bp. The Number Of Amplified Bands Varied From 12 In Primer 841 To 18 In Primer 881. The Maximum Percent Of Polymorphic Bands (84.6 %) Was Obtained Using Primer 857, Whereas The Minimum Percent Of Polymorphic Bands (27.8 %) Was Obtained In Primer 881 With An Average Value Of 51.78% Polymorphism. Average Number Of Bands And Polymorphic Bands Per Primer Were 14 And 7.25, Respectively. The Polymorphic Information Content (Pic) Value Ranged Between 0.29 And 0.76 With An Average Of 0.49. The Mean Nei?�?S Gene Diversity And Shannon?��?S Information Index Were 0.245 And 0.325, Respectively. Genetic Relationship Between A. Hypogaea Accessions On The Basis Of Jaccard?��?S Pair Wise Similarity Coefficients Varies From 44% To 83% With An Average Value Of 63.5%. The Upgma Dendrogram Based On Cluster Analysis Grouped The Forty Three A. H