Phytochemical Investigation And Antibacterial Studies Of Root Extract Of Gladiolus Candidus.

Abstract

Plants Have Been Used To Treat A Wide Range Of Diseases Throughout The History Of Human Beings. The Genus Gladiolus (Iridaceae), With Nearly 300 Species Confined Mainly To Africa, Has Proved To Be One Of The Most Important Sources Of Biologically Active Compounds. In Ethiopian The Root Of Gladiolus Candidus (Local Name Dallo In Afan Oromo And Milas Golgul In Amharic) Is Used For The Treatment Of Several Diseases Including Infectious Diseases And Cancer (Nekersa). This Study Focused On Extraction, Isolation And Characterization Of The Chemical Constituents Using Cc And Spectroscopic Method From The Roots Of Gladiolus Candidus And Testing Of Antibacterial Activities Of The Extracts And Purified Compounds. The Roots Were Successively Extracted Withn-Hexane, Ethyl Acetate And Methanol. The Ethyl Acetate Extracted, Followed By Separation Using Silica Gel Column Chromatography. That Afforded Two Compounds Characterized As , Isomerofisoflavenes3(3,4-Dihydroxyphenyl)-2h-Chromene-7,8-Dioland3-(6-Isopentylcyclohex-3-Enyl)2?��?Methylpropan1?��?-Ol).Phytochemical Screening Of The Methanol And Etoac Crude Extracts Of Root Of Gladiolus Candidus Revealed The Presence Of Flavonoids, Tannins, Alkaloids And Saponins. The Present Study Was Used Gravity Column With Ethyl Acetate And N-Hexane As An Eluent. However, Using The Solvent System Like N-Hexane: Etoac: Chcl3: Meoh Extracts, More Phytochemical Analysis Needs To Be Carried Out On These Polar Extracts Of The Plant. This Study Proved That The Crude Etoac Extract, Compound 30 And Compound 31 Have Promising Activity (12, 11 And 14 Mm Respectively) Against, Pseudomonas Aeruginosa, Which Might Give A Clue About The Traditional Use Of The Plant Against Various Infectious Diseases. Biological Assay On Other Strains Needs To Be Conducted On Future Work Of Extracts Of The Plant To Establish The Traditional Uses Of The Plant.