Investigation Of The Effects Of Silver/Zinc Oxide Nanocomposite Dispersed Modified Elastin-Collagen Matrix On The Mechanical ,Thermal And Antimicrobial Properties Of Poly (Lactic Acid)

Abstract

Poly(Lactic Acid) (Pla) Has Distinctive Characteristics, Including Biodegradability, Biocompatibility, Thermal Processability, High Transparency And Good Film Forming Ability. However Pla Has Some Poor Properties That Have Prevented Its Wide Applicability And These Properties Are Low Crystallization Rate, Poor Thermal Stability, And High Brittleness. The Main Objective Of This Research Was To Investigate The Effect Of The Modified Elastin-Collagen (M-Ell-Coll) Matrix And Silver/Zinc Oxide Dispersed Modified Elastin-Collagen (Ag/Zno/M-Ell-Coll) On Pla's Properties.The Ell Coll Matrix Was Extracted From Broiler Skin Waste And Modified By Grafting Using Lactic Acid Monomer To Facilitate Compatibility Issues With Pla. The Extracted And Modified Ell-Coll Matrix Were Investigated Using Ftir, And Both The Pre- And Post-Modification Persistence Of The ??-Helix And ??-Sheet Structures Were Confirmed. Chemical Methods Were Used To Synthesize Silver(Ag), Zinc Oxide (Zno) Nanoparticles (Nps), And Silver-Zinc Oxide Nanocomposite(Ag/Zno Ncs) And The Synthesized Nanomaterials Were Characterized Using Xrd, Ftir, Uv-Vis, And Psda. All The Characterized Nanomaterials Showed The Characterstics Peaks In Uv-Vis And Ftir Analysis. The Xrd Analysis Reveled That There Was No Additional Peaks Excepet For Characterstics Peaks Of Ag, Zno Nps And Ag/Zno Ncs Which Confirmed The Formation Of Pure Nanomaterials.Modified Elastin-Collagen Dispersed Poly(Lactic Acid) (Pla/M-Ell-Coll) Composite Films Were Prepared Using Solution Casting Method And Characterized Using Dsc And Utm. The Effect Of M-Ell-Coll As Nucleating Agent Resulted In Degree Of Crystallinity Of 58.8% With 10wt% M-Ell/Coll Loading, And The Elongation At Break Was Improved By 143.1 % For Pla/M-Ell-Coll-40% With Tensile Strength Of 33.75 Mpa Compared To Neat Pla. Following The Same Method Ag/Zno Dispersed M-Ell Coll/Pla Ternary Composite Films Were Prepared And Characterized Using Dsc, Tga And Disc Diffusion Method For Antimicrobial Activity. The Results Revealed That, All Ternary Composite Films Had Lower Thermal Stability Than Neat Pla Whereas Pla-Ell/Coll-Ag/Zno-10% Showed 43.3% Degree Of Crystalinity. For The Antibacterial Properties Of The Ternary Composite Film Pla-M-Ell/Coll Ag/Zno-10% Had The Largest Inhibition Zone, Which Was Approximately 12.5 Mm For Staphylococcus Aureus (Gram-Ve) And 13 Mm For Streptococcus Pyogenes (Gram+Ve) Bacteria Compared To Control (Ampicillin). As A Result, The Eco-Friendly Composite Films Could Be Widely Used In Different Area Of Applications Specially For Active Packaging Purposes.