In vitro protocol optimization for mass propagation of cooking banana (Musa Sapientum L.) (Matoke and Nijiru cultivars)

Abstract

Edible bananas (Musa spp.) are the major staple food for rural and urban consumers in the tropical and subtropical countries and an important source of rural income. The study was aimed to develop a simple, comprehensive and efficiently repetitive protocol for micropropagation of banana (Musa sapientum L.). For this experiment the banana cultivars used were Matoke and Nijiru. Those two cultivars were taken from the field and grew under green house for three month and taken to the laboratory for initiation. After two month of the culture initiation the developed culture shoot proceed to the shoot multiplication and rooting media. Using shoot meristem and increase the production and productivity of the farmers. The shoot tip initiation response survival of75% and 68% were obtained from explant cultured on MS medium supplemented with 3.0 mg/l BAP alone for both banana cultivars Matoke and Nijiru respectively. The highest number of shoots and shoot multiplication rate was obtained on MS medium supplement with a combination of BAP and IAA at concentrations of 3mlBAP+0.6ml IAA mg/l (14.2) for Matoke cultivar. For Nijiru cultivar most productive and produced maximum number of shoots at concentration of BAP and IAA; 3mlBAP+0.2mlIAA (11.6) followed by 3mlBAP+0.4mlIAA. Antibiotic (cefotaxime) was used to check the endogenously born bacterial contamination. The proliferated shoots were excised and transferred to different root induction media, which resultantly showed that MS media supplemented with IAA NAA and IBA, were the most efficient root inducing media. Rooted plantlets after primary and secondary hardening were transferred to the green house in which were successfully acclimatized.