Isolation And Structural Elucidation Of Compounds From The Root And Aerial Parts Of Dicliptera Laxata

Abstract

The present study was carried out on the phytochemical investigation , antimicrobial and antioxidant activities on the root and aerial parts of Dicliptera laxata (Acanthaceae), the plant is used for traditional medicine, especially avoids debility, headache and coughs. In the present study, the aerial and root parts of the plant were sequentially extracted with organic solvents. Phytochemical screening of the plant were performed on petroleum ether, chloroform, methanol, chloroform/methanol, and petroleum ether/ethyl acetate the aerial and root parts of crude extracts and showed that the presence of alkaloids, flavonoids, sterols, tannins and saponins compounds that has not been screened previously in plant species might be responsible for the claimed activities by local people. The volatile oil of leaf part of the plant was extracted by steam distillation. GC-MS of volatile oil of the aerial part of the plant showed the presence of 47 essential oil compounds with ≥70 % qualitative abundance and the % yield of 0 .23. In vitro antibacterial activities tests on crude extracts of the plant were done. There is no significant antibacterial effect on chloroform, methanol, chloroform/methanol, and petroleum ether/ethyl acetate aerial and root crude extracts with 0.5mg &1.5mg using disc diffusion method. The antioxidant activity test conducted on aerial chloroform extract indicated its antioxidant properties with % inhibition with DPPH = 55.4% of 12.5 mg/mL of sample concentration. Repeated use of solvent extraction followed by column chromatography of the plant aerial and root partsand yielded different compounds. Compound 1 was characterized as β- sitosterol and compound 2 was a heterocyclic crystalline compound which was partially characterized using UV, FT-IR and NMR spectroscopy by comparing with literature values.