Isolation, Molecular Detection and Antibiotic Susceptibility Patterns of Salmonella enterica serovars enteritidis and typhimurium from Adama City Butcher houses, Oromia regional state, Ethiopia

Abstract

Non-typhoidal Salmonella has been found to be the major cause of foodborne diseases and a serious public health problem in the world, with an increasing concern for the emergence and spread of antimicrobial resistant strains. The common Salmonella enterica serovars Enteritidis and Typhimurium represent the major serovars associated with human salmonellosis. Contamination of meat products with these serovars is considered the main source of infection. Therefore, this study is aimed to isolate and detect Salmonella enterica serovars enteritidis and typhimurium by molecular tool from Adama City butcher houses and to assess the antibiotics resistance profile of the isolates. A total of 48 samples consist of liver stomach tissue and raw meat samples were investigated for the presence of Salmonella species. The isolates were identified based on cultural, biochemical methods and polymerase chain reaction. The polymerase chain reaction was applied to test the presence of sdfI and spy specific target genes suspected to have a role in infection. In the present investigation, 7 isolates of Salmonella serovars were isolated from 48 bovine tissue samples (the raw meat, liver and stomach) collected from the butcher house of Adama city. From the total isolates of Salmonella serovars 5(71.43%) were Salmonella Typhimurium, while 2(28.57%) of the isolates were Salmonella Enteritidis which using the target genes by polymerase chain reaction. All Salmonella serovars were sensitive to Ciprofloxacin, Ceftraxene and Gentamycin. But 71.41% and 100% of the isolates were found resistant to Tetracycline and Azethromycin respectively. The commonly used antibiotics and misuse use of them may have contributed to the development of resistance to Salmonella serovars. Since the use of Ceftraxene, Gentamycin and Ciprofloxacin should be selected for treating Salmonella rather than Azethromycin and tetracycline. Therefore, further molecular characterization of pathogenic serovars with detection of antibiotics resistant gene and virulence gene can have paramount role for diagnosis of Salmonella direction for action to minimize food contamination and resistance gene development.