Synthesis Of Zinc Oxide Nanoparticles By Solo Chemical Method And Evaluation Of Its Antibacterial Activity On Selected Drug Resistant Bacteria

Abstract

Antibacterial Activity Of Zinc Oxide Nanoparticles Has Received Significant Interest Worldwide Particularly By The Implementation Of Nanotechnology To Synthesize Particles In The Nanometer Region. Therefore, The Objective Of This Study Is To Synthesize And Characterize Zinc Oxide Nps Having Wurtzite Crystalline Structure, And Study Its Anti-Bacteria Activity Against Some Selected Bacteria By Using Solochemical Method. Zinc Oxide Nps Were Synthesized Using Zn(Ch3coo)2.2h2o, Zncl2.6h2o, Zn(No3)2.6h2o And Naoh As Raw Materials, And Distilled Water And Ethanol Were Also Be Used As Solvents. The Synthesized Zno Nps Were Characterized By Thermal Gravimeter (Tga), X-Ray Diffraction (Xrd), Scanning Electron Microscopy (Sem), Uv-Visible Spectroscopy(Uv-Vis) And Fourier Transform Infrared Spectroscopy (Ft-Ir).The Xrd Analysis Has Provided Evidences That The Nps Obtained By Applying Zn(No3)2.6h2o And Zn(Ch3coo)2.2h2o ,As Precursors, Have Pure Hexagonal Wurtzite Crystalline Structure, Typical Of Zno, With Average Particle Sizes Of 21.62 Nm And 35.97 Nm, Respectively. The Variations In Sizes Of Zno Nps Are Due To The Difference In Precursors, Calcinations Temperatures, Exposure Time And Reaction Temperature.The Ft-Ir Analysis Indicates The Peaks Due To Vibrational Phonons Of Zno Nps Also Confirm The Successful Production Of Zno Nps By This Method.The Uvvis Absorbance /Reflectance Peak Variations Among The Zno Nps Are Due To The Difference In Their Size And Shape, Which Results Due To The Difference In Precursors And Calcination Temperatures. The Synthesized Zno Nps Was Applied And Compared For Antibacterial Activity Against Escherichia Coli, Basilius Subtilis, Pseudomonas Aeruginosa And Staphylococcus Aureus Bacteria Using Agar Well Diffusion Method. Among The Bacterial Classes Tested, Only The Grampositive Basilius Subtilis Were Found To Be Particularly Resistant To The Zno Nps Synthesized By This Method. This Study Also Revealed That The Zno Nps Synthesized From The Hydrated Forms Of Zinc Nitrate, Zinc Chloride And Zinc Acetate Have Budding Antibacterial Activity Against Escherichia Coli, Pseudomonas Aeruginosa And Staphylococcus Aureus Bacteria Because Znonps Exhibit Attractive Antibacterial Properties Due To Increased Specific Surface Area As The Reduced Particle Size Leading To Enhanced Particle Surface Reactivity.