Green Methods Of Synthesizing Zinc Oxide Nanoparticles Using Moringa Oleifera Leaf Extract For The Application Of Antimicrobial Activities

Abstract

In this study, the ZnO NPS were synthesised by dissolving 2 g and 4 g of zinc nitrate hexahydrate into 20 mL and 50 mL of extracted solution of moringa oleifera leaf powder by using Green synthesis method calcined at 400 0c and 500 0c respectively for antimicrobial applications. The average crystallite size of the ZnO Nps synthesized by dissolving 2 g of zinc nitrate hexa-hydrate into 20 mL 0f extracted solution of moringa oleifera leaf powder and 4 g of zinc nitrate hexa-hydrate by dissolving into 50 mL of extracted solution of moringa oleifera leaf powder determined by XRD were about 8.82 nm and 13.724 nm respectively. Additionally the ZnO NPS synthesized by dissolving each of 1 g and 3 g of zinc nitrate hexa-hydrate into 50 mL of extracted solution of moringa oleifera leaf powder calcined at a temperature of 500 .Their crystal sizes were about 11.57 nm and 12.62 nm respectively. The lattice parameters,a = 0.287 nm and c = 0.497 nm,ratio of c / a =1.731. The UV-Vis absorption peak of the ZnO NPs was observed at 364.2 nm and 370 nm for ZnO Nps synthesized by dissolving 2 g of zinc nitrate hexa-hydrate into 20 mL 0f extracted solution of moringa oleifera leaf powder and 4 g of zinc nitrate hexa-hydrate by dissolving into 50 mL of extracted solution of moringa oleifera leaf powder respectively. The band gap energy of samples M1 and M2 are 3.4 eV and 3.35 eV respectively. The vibrational phonon of ZnO NPS observed in the region of 433 cm -1 -510 cm -1 confirms the successful production of ZnO nanoparticles. The excitation wavelength of the ZnO NPS were synthesized by dissolving 2 g of zinc nitrate hexa-hydrate into 20 mL 0f extracted solution of moringa oleifera leaf powder and 4 g of zinc nitrate hexa-hydrate bydissolving into 50 mL of extracted solution of moringa oleifera leaf powder performed at 275 nm, 355 nm and respectively.The ZnO NPS having particle sizes of 8.82 nm and 13.724 nm were showed a larger inhibition zone on gram positive bacterial such as S.aureus and B.subtilis and a smaller inhibition zone on gram negative bacterial such as E.coli,P.aeurginosa,Typhus and C.albicans fungus. Among the two ZnO NPs, the ZnO NPs having particle size 8.882 nm showed an excellent antimicrobial activity on gram positive bacterial than gram negative bacterial and C.albicans fungus.