Biological And Chemical Synthesis Of Fe3o4 Nanoparticles And Evaluation Of It?�?s Anti bacterial Activity

Abstract

Iron oxide is interesting because it has vast applications in various areas. In this study iron oxide (Fe3O4) nanoparticles were synthesized by green method using 1:2 ratios of ferrous sulphate hepta hydrate and ferric chloride hexahydrate as precursory with the presence of hagenia abyssinica flower extract in different ratio and chemical method by using ferric nitrate and ethylene glycol. The synthesized Fe3O4 nanoparticles were characterized by x-ray diffraction (XRD), scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDS), ultraviolet visible spectroscopy (UV-Vis) and Fourier transformer infrared spectroscopy (FTIR). The crystal structure of nanoparticles were determined by XRD analysis and the particle size of the nanoparticles were in the range of 8 – 9.2 nm for green synthesized nanoparticles and 9.28nm for that of chemically synthesized nanoparticles.The shape of the nanoparticles and elemental composition were determined by SEM-EDS respectively. The UV-Vis investigation confirms the formation of iron oxide NPs i.e. the reflectance peak was exist at 324.4 nm for green synthesized nanoparticles and 328.5 nm for chemically synthesized nanoparticles. Fourier transformer infrared spectroscopy study confirmed the presence of amines, carboxylic acids, phenols and alcohols . The synthesized nanoparticles were tested for anti bacterial activity. The chemically synthesized Fe3O4 Nanoparticle showed a good antibacterial activity on E. coli and S. aureus bacterial strains.