Isolation, Characterization, Antibacterial And Antioxidant Activities Of The Roots Extract Of Cyphostemma Niveum

Abstract

Cyphostemma niveum (Vitaceae) is a plant traditionally used for the treatment of various ailments including wound healing and killing pest. The air dried roots powder (300g) of Cyphostemma niveum were extracted successively with hexane, ethyl acetate and methanol to furnish 1g (0.33%), 5.1g (1.7%) and 20g (6.7%) respectively. The EtOAc extract after silica gel column chromatography has led to the isolation of two compounds coded as CN02 and CN06. The structure elucidations of these compounds were undertaken using spectroscopic techniques (UV, IR and NMR). The methanol, EtOAc extract and isolated compounds were assessed for their antibacterial activity against S. aureus, E. coil, P. mirabilis and K. pneumonia. Results showed that CN02 displayed activity against all bacterial pathogens tested in this study while the methanol extract was found active only against S. aureus. Furthermore, the antioxidant activities of the extracts were evaluated using DPPH. The MeOH extract displayed modest DPPH radical scavenging activity compared with ascorbic acid.