Green Synthesis of Fe2O3 and Fe3O4 Nps Using Calpurnia Aurea LeafExtract and Its Application for Antibacterial Activity

Abstract

Metal Oxide Nanoparticles have attained a great importance due to their unique properties. Among the metal oxide nanoparticles, Iron oxide is interesting because it has vast applications in various areas. In this study comparing iron oxide Fe2O3 and Fe3O4 nanoparticles. The Fe2O3 were synthesized by biogenic method using FeCl3.6H2O and again Fe3O4 nanoparticles were synthesized by biogenic method using 1:2 ratios of ferrous sulphate hepta hydrate and ferric chloride hexa hydrate as precursory with the presence of C.aurea leaf extract in ratio of (2:1,1:2,1:1) for both of them . The synthesized nanoparticles were tested for antibacterial activity. The synthesized Fe2O3 and Fe3O4 nanoparticles were characterized by x-ray diffraction (XRD), scanning electron microscopy-energy, ultraviolet visible spectroscopy (uv-vis) and Fourier transformer infrared spectroscopy (FTIR). The crystal structure of nanoparticles were determined by XRD analysis the particle size of Fe2O3 nanoparticles 11.9,11.5 and 11.3nm for 2:1,1:2,1:1 ratio respectively and the particle size of Fe3O4 nanoparticles are 9.8,5.8 and 3.8nm for 2:1,1:2,1:1 ratio respectively. The DRS spectra investigation confirms the formation of Fe2O3 nanoparticles of the reflectance was exist at 200nm-850nm and for Fe3O4 nanoparticles 350nm- 800 nm. Fourier transformer infrared spectroscopy study confirmed the presence of amines, carboxylic acids, phenols and alcohols. Generally, magnetite nanoparticles were found to be a better antibacterial activity compared to hematite nanoparticles. Among the magnetite nanoparticles ratios 1:1 Fe3O4 exhibited the highest inhibition zone against P. aeruginosa (10.34±0.47mm) at the concentration of 300mg/ml. The lowest zone of inhibition against E. coli and P. aeruginosa was observed at a concentration of 18.75 mg/ml (6.34 ± 0.47 mm) with mean and standard deviation respectively. In contrast, the highest zone of inhibition against S. aureus was observed with hematite 1:1 Fe2O3 at a concentration of 300 mg/ml (10.33 ± 0.47 mm) and the lowest at the concentration of 18.75mg/ml (6.33±0.47mm) for the S. pyogens.