Development of Hybrid Biobased Ointment Matrix: The Effect of Ocimum Lamiifolium on its Antimicrobial Property

Abstract

An Ointment Is A Homogeneous, Viscous, Semi-Solid Preparation Intended For External Use On The Skin Or Mucous Membranes. Numerous Researches Were Done On The Formulation Of Herbal Ointment Taking Petroleum Derivatives As Ointment Bases To Carry Active Components Of Plant Extract. However, These Petroleum Derivatives Ointments Have A Negative Impact On The Skin Due To A Compatibility Problem. Chronically It Clogged The Skin Pores Which Subsequently Affects The Release Of Moisture. To Mitigate This Problem, A Novel Approach Has Been Followed To Synthesize A Completely Green And Biocompatible Ointment Base From Elastin And Collagen Which Are Naturally Existed In Our Skin And Lactic Acid Commonly Known As A Part Of Body Fluid. The Synthesis Of L-Lactic Acid Oligomer (Olla) And Lactic Acid Modified Elastin/Collagen (Olla-M-Eln/Col) Copolymer Was Conducted By An In-Situ Poly condensation Reaction. Different Grades Of Olla-M-Eln/Col Were Prepared By Varying Eln/Col Loading That Was Used For Carrying Plant Extract. The Six-Mass Loss In The Eln/Col Matrix Was Not Seen In The Synthesized Ointment Base. The Reason For Showing Similar Degradation Characteristics Might Be Due To Secondary Week Interaction That Happened Between The Carboxyl Group Of Lactic Acid Oligomer And The Amide Group Of Eln/Col Matrix. This Phenomenon Is Supported By The Formation Of A Broad Shoulder Like A Peak At 1745 Cm-1 With The Addition Of Elastin/Collagen Composition Within The Lactic Acid Oligomer. By Mimicking The Traditional Medicine Preparation Technique And Delivery Approaches Used For Skin-Related Diseases, Ocimum Lamiifolium (????????????) Was Chosen For Its Antimicrobial Effect. Formulations Of Antimicrobial Ointment Were Prepared At Different Concentrations Of Plant Extract By Fusion Method. These Topical Formulations And The Synthesized Ointment Bases Were Tested For Ph, Viscosity, Spreadability, Organoleptic Characteristics, And Homogeneity, And Antimicrobial Activity. The Synthesized Ointment Base Showed An Inhibition Zone Of 14mm And 12 Mm Respectively For S. Aureus And E. Coli. As Compared To A Positive Control (Which Is A General Antibiotic, Ampicillin), This Thesis Shows The Highest Zone Of Inhibition Against S. Aureus (25 Mm) And E. Coli (23 Mm) For The Dispersion Of 10% Ol In Eln/Col-Olla Bioconjugate Matrix. Therefore, This Research Work Concludes That The Synthesized Bioconjugate Can Be Used As A Base Matrix For The Formulation Of Different Herbal Biocompatible Ointments Targeting Skin Care Applications.